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Percentage of hypo-haemoglobinised red cells (HYPO-He) and hyper-haemoglobinised red cells (HYPER-He)

What are HYPO-He and HYPER-He used for?

Together with other parameters, HYPO-He is used in RBC indices to distinguish between iron defiency anaemia and thalassaemia.

The use of HYPO-He as well as MicroR is recommended in nephrology guidelines such as the European Best Practice Guidelines (EBPG), National Kidney Foundation Kidney Disease Outcome Quality Initiative (NKF KDOQI).

How are HYPO-He and HYPER-He determined?

HYPO-He and HYPER-He are parameters analysed in the reticulocyte (RET) channel. They are derived from the haemoglobin content of all mature RBC (RBC-He), which can be calculated based on the high-angle forward scatter (FSC). The FSC signal is converted into picograms (pg) using a proprietary algorithm and in healthy individuals the resulting RBC-He value is comparable to the mean corpuscular haemoglobin (MCH) value. Subsequently, this RBC-He value is used to determine HYPO-He and HYPER-He.

HYPO-He is the percentage of RBC with cellular haemoglobin content lower than 17 pg, whereas HYPER-He is the percentage of RBC with cellular haemoglobin content higher than 49 pg.



HYPO-He and HYPER-He are diagnostic parameters on XN-Class analysers that have the RET channel.


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